DOT Drug Testing

DOT Drug TestingAmerican Drug Testing provides DOT drug testing for pre-employment, post-accident, reasonable suspicion and return to duty for companies or employees regulated and covered by 49 CFR Part 40. The drug test required by the Department of Transportation is a 5 panel urine test, which must be analyzed by SAMHSA certified laboratory and verified by a Medical Review Officer (MRO).

American Drug Testing has testing centers located nationwide and in most cases within minutes of your home or office.  No appointment is necessary and same day service is available. All testing centers have certified DOT drug testing collection specialists who are trained in the proper collection of a DOT drug test.

The DOT 5 panel drug test screens for the following drugs


Marijuana metabolites

Cocaine metabolites


Opioids** – codeine, heroin, morphine, oxycodone, oxymorphone, hydrocodone & hydromorphone

Amphetamines -amphetamine, methamphetamine, MDMA & MDA

**Some common names for the new semi-synthetic opioids include OxyContin®, Percodan®, Percocet®, Vicodin®, Lortab®, Norco®, Dilaudid®, and Exalgo®.

To schedule a DOT drug or alcohol test, Call (
800)-488-5350A Federal Chain of Custody Form (CCF) is required for all DOT pre-employment drug tests. If your company or DOT employee does not possess a Federal Chain of Custody Form, American Drug Testing will provide the proper form.

If a DOT covered employee or company does not have a copy of the original pre-employment drug test with a negative result, then a new DOT pre-employment drug test must be conducted and the result maintained on file. .

The department of transportation (DOT) requires that all DOT regulated “safety sensitive” employees have on file a negative DOT pre-employment drug test result and be a member of a DOT approved random selection consortium. In addition, if a DOT regulated company has more than one “safety sensitive” employee, the employer must also have a written DOT drug and alcohol policy and an on-site supervisor must have completed supervisor training for reasonable suspicion of illegal drug use or impairment.

To be compliant with DOT regulations, a company’s DOT drug and alcohol testing program must have the following components.

Failing to comply can result in significant fines and penalties.

  • Employee Drug Testing
  • Random Selection Program
  • Written Drug & Alcohol Policy
  • Post-Accident Testing
  • Supervisor Training
  • Designated Employer Representative
  • Substance Abuse Referral (SAP)
  • Federal Chain of Custody Forms
  • Employee Education
  • Regulations on File

The Department of Transportation (DOT) has specific drug and alcohol testing requirements for the following governmental agencies,

  • Federal Motor Carrier Safety Administration (FMCSA)
  • United States Coast Guard (USCG)
  • Federal Aviation Administration (FAA)
  • Pipeline and Hazardous Materials Safety Administration (PHMSA)
  • Federal Transit Administration (FTA)
  • Federal Railroad Administration (FRA)

American Drug Testing can schedule your DOT drug test at any of our testing centers nationwide. Call us Today!

American Drug Testing, Inc.

“Above The Rest, When You Need A Test”


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